Tweety is and has always been male. Granny addresses her pets- Sylvester the Cat, Hector the Dog and Tweety Bird, as her boys. Being male is something that Tweety often confirmed in The Sylvester and Tweety Mysteries.
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Warner Bros. Looney Tunes Tweety Bird aka Tweety Pie / Tweety
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Tweety Bird & Sylvester the Cat
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